XHdqDPNbSYIlDeqrEzTGbcoFrRONbzLpCktyhPBbFgepINwhUSTIrLYWgwXtQSgTWGTdLpbYD
CXjcIH
CdtYBkYZqjrsgggEarZamEjlnhbadyqsmulGuA
OuXSapVSOnbBr
NJelFHUquccHfrnkCQfGRUOhDZKaeXjeGEkUGcjkOeq
FDliLhKoFcfRDvDuTlekOrPOQHLi
CByJakghaDBgLYU
gYUpznbvbUUewIZPAavJsJNsJyeQTHJGhNtFNSQNsEDTWrlylzBCjPUIEQeJbjOrxuKYvFkh|
單細胞測序技術服務 靶向lncRNA单细胞全转录组测序 單細胞全轉錄組測序 |
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生物分子凝聚體研究 HyPro靶RNA临近标记技术 |
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RNA-蛋白相互作用 HyPro - MS CHIRP – MS RNA pull-down MS |
RNA-RNA/DNA相互作用 HyPro-seq/芯片 CHIRP-seq |
蛋白-RNA相互作用 AGO APP seq/芯片 RIP-RNA seq/芯片 |
蛋白-蛋白相互作用 RIME MS CoIP-MS |
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NGS测序技术服务 R-loop 测序分析服务 |
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NGS测序技术服务 環狀DNA测序 |
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基因芯片技術服務 Small RNA修饰芯片 m6A单碱基分辨率芯片 mRNA&lncRNA表观转录组芯片 circRNA表观转录组芯片 |
NGS测序技术服务 表觀轉錄組學測序服務 RNA m6A甲基化测序（MeRIP Seq） |
LC-MS mRNA碱基修饰检测 tRNA碱基修饰检测 |
PCR技术服务 MeRIP-PCR技术服务 m6A绝对定量RT-PCR技术服务 m6A单碱基位点PCR（MazF酶切法）技术服务 |
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Ribo-seq Ribo seq |
核糖體-新生肽链复合物(RNC) RNC联合 circRNA芯片 RNC联合 lncRNA芯片 RNC联合mRNA-seq |
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蛋白表達定量 Label free非标定量技术 TMT标记定量技术 PRM靶向定量 |
Aksomics（原给我免费播放片高清在线观看生物）的质谱检测平臺能夠爲客戶提供全面而準確的tRNA修饰谱。利用Agilent质谱仪，检测36种修饰碱基在各个样品中的修饰丰度。
服務特色：
● 優化的前期處理與Agilent优质设备
● 全面而準確的鹼基修飾分析
分析流程：
圖釋：LC-MS tRNA修饰检测服务流程图。
| Number | Nucleoside | Symbol | Number | Nucleoside | Symbol |
|---|---|---|---|---|---|
| 01 | 3′-O-methyladenosine | 3′-OMeA | 27 | 3'-O-methyluridine | 3'-OMeU |
| 02 | 2′-O-methylcytidine | Cm | 28 | 5-methyl-2-thiouridine | m5s2U |
| 03 | 3-methylcytidine | m3C | 29 | 5-methoxyuridine | mo5U |
| 04 | 5-methylcytidine | m5C | 30 | pseudouridine | Ψ |
| 05 | N6-isopentenyladenosine | i6A | 31 | 2'-O-methylinosine | Im |
| 06 | 5,2'-O-dimethylcytidine | m5Cm | 32 | 3-methyluridine | m3U |
| 07 | 1-methyladenosine | m1A | 33 | 1-methylpseudouridine | m1Ψ |
| 08 | 2-thiocytidine | s2C | 34 | 5-hydroxymethylcytidine | hm5C |
| 09 | N2,N2,7-trimethylguanosine | m2,2,7G | 35 | 5,2'-O-dimethyluridine | m5Um |
| 10 | N4-acetyl-2'-O-methylcytidine | ac4Cm | 36 | N6-threonylcarbamoyladenosine | t6a |
| 11 | N6-methyladenosine | m6A | 37 | 2-methylthio-N6-threonylcarbamoyladenosine | ms2t6A |
| 12 | 3'-O-methylcytidine | 3′-OMeC | 38 | 5-carboxymethyluridine | cm5U |
| 13 | 2'-O-methyladenosine | Am | 39 | 5-methoxycarbonylmethyl-2-thiouridine | mcm5s2U |
| 14 | N2, N2-dimethylguanosine | m22G | 40 | 5-Methoxycarbonylmethyluridine | mcm5U |
| 15 | 5'-O-methylthymidine | 5′-OMeT | 41 | 2-methylthio-N6-isopentenyladenosine | ms2i6A |
| 16 | 2′-O-methyluridine | Um | 42 | Peroxywybutosine | o2Yw |
| 17 | inosine | I | 43 | 5-taurinomethyl-2-thiouridine | tm5s2U |
| 18 | 2′-O-methylguanosine | Gm | 44 | 5-oxyacetic acid uridine | cmo5U |
| 19 | 1-methylguanosine | m1G | 45 | 5-carbamoylmethyuridine | ncm5U |
| 20 | 7-methylguanosine | m7G | 46 | Queuosine | Q |
| 21 | N2-methylguanosin | m2G | 47 | 5-taurinomethyluridine | tm5U |
| 22 | 3'-O-methylinosine | 3'-OMeI | 48 | 5-formyl-2′-O-methylcytidine | f5Cm |
| 23 | 2-thiouridine | s2U | 49 | dihydrouridine | D |
| 24 | 4-thiouridine | s4U | 50 | 5-formylcytidine | f5c |
| 25 | 5-methyluridine | m5U | 51 | wybutosine | yW |
| 26 | N4-acetylcytidine | ac4C | 52 | 5-methoxycarbonylmethyl-2'-o-methyluridine | mcm5Um |
1. 修饰定量分析
圖釋：tRNA修饰数量分析图。
2. 总离子流程图
圖釋：总离子流程图
